Drosophila neuroblasts retain the daughter centrosome
نویسندگان
چکیده
During asymmetric mitosis, both in male Drosophila germline stem cells and in mouse embryo neural progenitors, the mother centrosome is retained by the self-renewed cell; hence suggesting that mother centrosome inheritance might contribute to stemness. We test this hypothesis in Drosophila neuroblasts (NBs) tracing photo converted centrioles and a daughter-centriole-specific marker generated by cloning the Drosophila homologue of human Centrobin. Here we show that upon asymmetric mitosis, the mother centrosome is inherited by the differentiating daughter cell. Our results demonstrate maturation-dependent centrosome fate in Drosophila NBs and that the stemness properties of these cells are not linked to mother centrosome inheritance.
منابع مشابه
Cnn Dynamics Drive Centrosome Size Asymmetry to Ensure Daughter Centriole Retention in Drosophila Neuroblasts
Centrosomes comprise a pair of centrioles surrounded by an amorphous network of pericentriolar material (PCM). In certain stem cells, the two centrosomes differ in size, and this appears to be important for asymmetric cell division [1, 2]. In some cases, centrosome asymmetry is linked to centriole age because the older, mother centriole always organizes more PCM than the daughter centriole, thu...
متن کاملThe Centriolar Protein Bld10/Cep135 Is Required to Establish Centrosome Asymmetry in Drosophila Neuroblasts
Centrosome asymmetry has been implicated in stem cell fate maintenance in both flies and vertebrates [1, 2]. Drosophila neuroblasts, the neural precursors of the fly's central nervous system [3], contain molecularly and physically asymmetric centrosomes, established through differences in pericentriolar matrix (PCM) retention [4-7]. For instance, the daughter centriole maintains PCM and thus mi...
متن کاملExtrinsic cues orient the cell division axis in Drosophila embryonic neuroblasts.
Cell polarity must be integrated with tissue polarity for proper development. The Drosophila embryonic central nervous system (CNS) is a highly polarized tissue; neuroblasts occupy the most apical layer of cells within the CNS, and lie just basal to the neural epithelium. Neuroblasts are the CNS progenitor cells and undergo multiple rounds of asymmetric cell division, ;budding off' smaller daug...
متن کاملPLP helps the mother centrosome stay mum
PLP helps the mother centrosome stay mum D rosophila neuroblasts give rise to the fl y's central nervous system by dividing asymmetrically to produce a daughter neuroblast and a gan-glion mother cell (GMC). The neuroblast's centrosomes are themselves asymmetric in terms of their activity, localization, and pattern of inheritance. Lerit and Rusan describe how the centrosomal protein pericentrin ...
متن کاملDrosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules
Disruption of the function of the A-type Aurora kinase of Drosophila by mutation or RNAi leads to a reduction in the length of astral microtubules in syncytial embryos, larval neuroblasts, and cultured S2 cells. In neuroblasts, it can also lead to loss of an organized centrosome and its associated aster from one of the spindle poles, whereas the centrosome at the other pole has multiple centrio...
متن کامل